PE / R-Phycoerythrin Conjugation Kit - Lightning-Link from Abcam

Product Specs
ItemPE / R-Phycoerythrin Conjugation Kit - Lightning-Link
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab102918
Quantity1 X3MG, 3 X10UG, 3 X60UG, 5 X600UG, 60 UG, 600 UG
ApplicationsConjugation
TargetPE

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Antigen density quantification of cell-surface immunotherapy targets by flow cytometry: Multi-antigen assay of neuroblastoma bone marrow metastasisSTAR ProtocolsDecember 1, 2023Molly T. Radosevich, Scott J. Bornheimer, Majid Mehrpouryan, Bita Sahaf, Jean S. Oak, Crystal L. Mackall, Sabine Heitzenederproteins","Chemicals, peptides, and recombinant proteins" ,, Abcam PE Conjugation Kit Lightning-Link,Abcam,Cat# ab102918 Abcam APC Conjugation Kit Lightning-Link,Abcam,Cat# ab201807 BD Quantibrite PE beads,BD Biosciences,Cat#

(2) Spatial transcriptomic profiling of coronary endothelial cells in SARS-CoV-2 myocarditisFrontiers in MedicineMarch 9, 2023Camilla Margaroli, Paul Benson, Maria G. Gastanadui, Chunyan Song, Liliana Viera, Dongqi Xing, J. Michael Wells, Rakesh Patel, Amit Gaggar, Gregory A. PayneAnti-SARS-CoV-2 was conjugated to PE/R-Phycoerythrin (Expedeon Lightning-Link R-PE Conjugation Kit/Abcam, ab102918), while anti-CD31 was linked to Alexa Fluor 488 (Abcam, Lighting-Link ab236553). Nuclei were counterstained

(3) Direct selection of functional fluorescent-protein antibody fusions by yeast displayPLOS ONEFebruary 24, 2023Nileena Velappan, Fortunato Ferrara, Sara D’Angelo, Devin Close, Leslie Naranjo, Madeline R. Bolding, Sarah C. Mozden, Camille B. Troup, Donna K. McCullough, Analyssa Gomez, et al.anti-SV5 (ThermoFisher #37–750, 1μg/mL) conjugated with phycoerythrin (PE) using a commercial kit (Abcam #ab102918), and Alexa 633-labeled streptavidin (ThermoFisher Scientific, S21375, 5 μg/mL) for 30 minutes. The

(4) LRRC15 mediates an accessory interaction with the SARS-CoV-2 spike proteinPLoS biologyFebruary 1, 2023Shilts J, Crozier TWM, Teixeira-Silva A, Gabaev I, Gerber PP, Greenwood EJD, Watson SJ, Ortmann BM, Gawden-Bone CM, Pauzaite T, et al.#71505) and then covalently conjugated to R-phycoerythrin using a commercial amine coupling kit (Abcam #ab102918). Transfected HEK293 cells were transferred to v-bottom 96-well plates (Greiner #651261). Comparatively

(5) A Spike-destructing human antibody effectively neutralizes Omicron-included SARS-CoV-2 variants with therapeutic efficacyPLoS pathogensJanuary 1, 2023Meng L, Zha J, Zhou B, Cao L, Jiang C, Zhu Y, Li T, Lu L, Zhang J, Yang H, et al.SARS-CoV-2 RBD (Cat: 40592-V08B, Sinobiological) was conjugated with FITC (Cat: ab102884) and (Cat: ab102918) Lightning-Link Conjugation Kits, respectively, according to the manufacture’s procedure (Abcam). (Isolation)

(6) BNT162b2-induced neutralizing and non-neutralizing antibody functions against SARS-CoV-2 diminish with ageCell reportsOctober 25, 2022Bates TA, Lu P, Kang YJ, Schoen D, Thornton M, McBride SK, Park C, Kim D, Messer WB, Curlin ME, et al.Systems,Cat#9595-CD Recombinant FcγRIIb/CD32b,R&D Systems,Cat#1875-CD PE/R-Phycoerythrin Conjugation Kit,Abcam,Cat# ab102918 EZ-Link™ Sulfo-NHS-LC-Biotin,Thermo Scientific,Cat#21335 Streptavidin Magnetic Beads,New England Biolabs,Cat#S1420S PureProteome™ Protein G Magnetic Bead System

(7) IL-1Î²â€“dependent extravasation of preexisting lung-restricted autoantibodies during lung transplantation activates complement and mediates primary graft dysfunctionJournal of Clinical InvestigationOctober 17, 2022Wenbin Yang, Emily Jeong Cerier, FÃ©lix L. NÃºÃ±ez-Santana, Qiang Wu, Yuanqing Yan, Chitaru Kurihara, Xianpeng Liu, Anjana Yeldandi, Nigar Khurram, Diego Avella-Patino, et al.antibody was conjugated to R-PE using a PE/R-Phycoerythrin Conjugation Kit - Lightning-Link (Abcam, ab102918). Arterial blood gases Arterial blood gases were measured using a fraction of inspired oxygen of 100%

(8) A covalent inhibitor of K-Ras(G12C) induces MHC class I presentation of haptenated peptide neoepitopes targetable by immunotherapyCancer cellSeptember 12, 2022Zhang Z, Rohweder PJ, Ongpipattanakul C, Basu K, Bohn MF, Dugan EJ, Steri V, Hann B, Shokat KM, Craik CS.assays,Critical commercial assays,Critical commercial assays LightningLink-PE,Expedeon/Abcam,Cat. No. ab102918 LightningLink-HRP,Expedeon/Abcam,Cat. No. ab102890 LightningLink-Dylight 800,Expedeon/Abcam,Cat. No.

(9) Impact of CTLA-4 checkpoint antibodies on ligand binding and TransendocytosisFrontiers in ImmunologyAugust 31, 2022Cayman Williams, Alan Kennedy, Maximillian A. Robinson, Christopher Lloyd, Simon J. Dovedi, David M. SansomFree Hospital pharmacy. Fluorochrome conjugation was carried out using the PE/R-Phycoerythrin (Cat# ab102918) or APC (Cat# ab201807) conjugation kit - Lightning-link (Abcam) according to manufacturers instructions

(10) Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanismImmunityJuly 12, 2022Gregoire C, Spinelli L, Villazala-Merino S, Gil L, Holgado MP, Moussa M, Dong C, Zarubica A, Fallet M, Navarro JM, et al.peptides, and recombinant proteins" ,, PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®,Abcam,Cat# ab102918 APC Conjugation Kit - Lightning-Link®,Abcam,Cat# ab201807 Alexa Fluor® 700 Conjugation Kit (Fast) - Lightning-Link

(11) Targeting human CALR‐mutated MPN progenitors with a neoepitope‐directed monoclonal antibodyEMBO reportsApril 5, 2022Denis Tvorogov et al.TpoR, CALRWT and mutant CALR were incubated for 30 min 4D7 conjugated to phycoerythrin (PE; Abcam, #AB102918) or IgG2a PE isotype control (ThermoFisher #12‐4321‐42) at the concentrations specified after pre‐incubation

(12) Antibody-drug conjugate efficacy in neuroblastoma - role of payload, resistance mechanisms, target density, and antibody internalizationMolecular Cancer TherapeuticsNovember 1, 2021Samantha Buongervino, Maria V. Lane, Emily Garrigan, Doncho V. Zhelev, Dimiter S. Dimitrov, Kristopher R. Bosseinstructions. The D3-GPC2-IgG1 antibody was conjugated to PE using a PE/R-Phycoerythrin Conjugation Kit (Abcam, ab102918). Western blotting Whole-cell lysates were prepared with cell lysis buffer (Cell Signaling Technology,

(13) Leukotriene B4 receptor-2 contributes to KRAS-driven lung tumor formation by promoting interleukin-6-mediated inflammationExperimental & molecular medicineOctober 1, 2021Jang JH, Park D, Park GS, Kwak DW, Park J, Yu DY, You HJ, Kim JH.Farmingdale, NY, USA) were conjugated with FITC and PE, respectively, using a conjugation kit (ab188285, ab102918, Abcam). The patient samples were deparaffinized, rehydrated, and blocked with buffer (PBS containing

(14) Ex vivo SARS-CoV-2 infection of human lung reveals heterogeneous host defense and therapeutic responsesJCI insightSeptember 22, 2021Schaller MA, Sharma Y, Dupee Z, Nguyen D, Urueña J, Smolchek R, Loeb JC, Machuca TN, Lednicky JA, Odde DJ, et al.temperature. Antibodies against viral proteins were directly conjugated using antibody labeling kits (ab102918; Abcam). For viability staining, a live/dead kit (R37601; Thermo Fisher Scientific) with Calcein AM

(15) IgG Immune Complexes Inhibit Naïve T Cell Proliferation and Suppress Effector Function in Cytotoxic T CellsFrontiers in ImmunologyAugust 10, 2021Wissam Charab et al.expressed. For staining, the 2B6 antibody was conjugated to PE (or FITC) using a dye conjugation kit (ab102918, Abcam). VL and VH sequences for the utilized TNP antibodies (clone 7B4), TYRP1 (Clone 20D7S) antibodies,

(16) A GPC2 antibody-drug conjugate is efficacious against neuroblastoma and small-cell lung cancer via binding a conformational epitopeCell reports. MedicineJuly 20, 2021Raman S, Buongervino SN, Lane MV, Zhelev DV, Zhu Z, Cui H, Martinez B, Martinez D, Wang Y, Upton K, et al.instructions. The D3-GPC2-IgG1 antibody was conjugated to PE using a PE/R-Phycoerythrin Conjugation Kit (Abcam, ab102918). Other primary antibodies utilized were as follows: APC anti-human CD117 (c-kit) antibody (clone 104D2,

(17) A Simple and Quick Method for Loading Proteins in Extracellular VesiclesPharmaceuticals (Basel, Switzerland)April 13, 2021Busatto S, Iannotta D, Walker SA, Di Marzio L, Wolfram J.(Thermo Fisher Scientific, Waltham, MA, USA) and PE/R-Phycoerythrin (R-PE) Conjugation Kit—Lightning-Link (ab102918) (Abcam, Cambridge, MA, USA) following the manufacturer’s instructions. 3.6. PULSin/Protein Nanoparticles PULSin

(18) NLRP3 inflammasome induces CD4+ T cell loss in chronically HIV-1–infected patientsJournal of Clinical InvestigationMarch 15, 2021Chao Zhang et al.with PE 10 fluorochromes using commercial conjugation kits according to the manufacturer’s protocols (AB102918, Abcam). Information on the antibodies and reagents used in this study is listed in Supplemental Table

(19) Pre-existing immunity to influenza virus hemagglutinin stalk might drive selection for antibody-escape mutant viruses in a human challenge modelNature MedicineAugust 1, 2020Jae-Keun Park et al.globular head, was conjugated with R-phycoerythrin (R-PE) using an R-PE Conjugation Kit (catalog no. Ab102918, Abcam). Anti-influenza nucleoprotein (NP) antibody (catalog no. MAB8257, Millipore Sigma) was conjugated

(20) β3-Adrenoreceptor Blockade Reduces Hypoxic Myeloid Leukemic Cells Survival and ChemoresistanceInternational journal of molecular sciencesJune 12, 2020Calvani M, Dabraio A, Bruno G, De Gregorio V, Coronnello M, Bogani C, Ciullini S, Marca G, Vignoli M, Chiarugi P, et al.cytofluorimetric analysis was obtained from ABCAM and conjugated with the R-Phycoerythrin Conjugation Kit (ab102918, ABCAM). Expression levels were obtained by using a MACSQuant Analyzer 10 flow cytometry. Apoptosis

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link from Abcam

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PE / R-Phycoerythrin Conjugation Kit - Lightning-Link from Abcam

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